Cisplatin a549

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August undefined, 2024

WebOct 1, 2024 · Cisplatin-resistant A549 cells (A549/CisR) were derived from A549 cells by continuous cisplatin treatment. The original parental A549 cells were initially treated … WebJan 1, 2024 · Results: Hypoxia exacerbated the cisplatin resistance in lung cancer cells due to the increased expression of PKM2 that was observed in the exosomes secreted by hypoxic cisplatin-resistance cells. We identified that hypoxia-induced exosomal PKM2 transmitted cisplatin-resistance to sensitive NSCLC cells in vitro and in vivo.

Targeted therapy for cisplatin‐resistant lung cancer via …

WebApr 8, 2024 · A549 cells were seeded at a density of 3×10 4 cells/well for the C group (sensitive cells) and 9×10 4 cells/well for the CIS population (resistant cells) in 96-well … WebOur results identified cisplatin resistant subpopulations of NSCLC cells with a putative stem-like signature, providing a further understanding of the cellular events associated with the cisplatin resistance phenotype in lung cancer. csrf_field laravel 8

Cisplatin and phenanthriplatin modulate long-noncoding RNA …

WebJul 26, 2024 · The efficacy of cisplatin-based chemotherapy in cancer is limited by the occurrence of innate and acquired drug resistance. In order to better understand the … Webined if clusterin expression affects the cisplatin sensitivity in vivo; A549, A549/sCLU, and A549/pcDNA3.1 cells were injected subcutaneously into the right flank of nude mice. Cisplatin could significantly inhibit the tumor growth in mice injected with A549/pCDNA3.1 scramble cells and A549 cells compared to the mice injected with Webined if clusterin expression affects the cisplatin sensitivity in vivo; A549, A549/sCLU, and A549/pcDNA3.1 cells were injected subcutaneously into the right flank of nude mice. … eaoron makeup remover balm

Cisplatin-resistant A549 non-small cell lung cancer cells …

Reversal of resistance to cisplatin in NSCLC by miRNA-195-5p

WebAug 15, 2005 · Both BSO and siRNA sensitized melphalan and cisplatin to a small extent . In A549 cells, the GSH level was reduced to 43 ± 2% of the control in the presence of 10 μmol/L of BSO. Therefore, GSH-mediated detoxification seems to play various roles with all the drugs tested in this study, except for l-alanosine. The geldanamycin analogues ... WebFeb 28, 2024 · The approximately 85% of lung cancers are non-small cell lung cancer (NSCLC), with dismal median overall survival and 5-year survival rates due to recurrence, extensive invasion and metastasis 2-4. Cisplatin (DDP) is a first-line cytotoxic agent for the treatment of human NSCLC. csrf golangWebNov 29, 2024 · This study revealed that cisplatin resistant A549 lung cancer cells can be identified by their increased levels of mitochondrial mass. However, Mito-High cells … csrf get local storage

"WebCisplatin (DDP) has been a significant tumor chemotherapy against various solid malignant tumors, including lung adenocarcinoma, since it was approved by the Food and Drug … " - Cisplatin a549

Cisplatin a549

IC 50 values for cisplatin, curcuminoid, and combination …

WebJan 12, 2024 · To evaluate whether cilastatin influences the antitumor effects of cisplatin in human cancer cells, SCID mice were injected with A549 human lung adenocarcinoma cells. The mice were treated with... WebFeb 28, 2024 · All of these new complexes have been tested on three human cancer cell lines (A549 lung, SKOV3 ovarian, and MCF-7 breast cancer cell lines) and studied for …

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WebThe 50 % inhibitory concentration (IC50) of cisplatin in A549 and A549/DDP cells 48 h after drug treatment Source publication +4 RNA interferences targeting the Fanconi … WebIn addition, GA reduced the MRP2 and LRP protein expression level in A549/DDP cells. GA inhibits the proliferation, induces cell cycle arrest and apoptosis in A549/DDP cells. Combination of GA with cisplatin enhances the antitumor effects on cisplatin-resistant lung cancer cells by downregulating MRP2 and LRP expression.

WebEffect of MiR-195-5p on the Sensitivity of A549 and A549/DDP Cells to Cisplatin Sensitivity to cisplatin in the different groups was measured as the IC 50 concentration after transfection. Growth inhibition rate of the A549 cells was 52.0% under the effect of cisplatin at an IC 50 concentration of 0.8 μg/mL but decreased to 37.6% after ... WebSilencing USP14 increased the tumor antagonistic action of DDP in A549 cisplatin-resistant (A549/DDP) cells, while USP14 overexpression decreased the antagonist effects. Aptamer-targeted nano-zinc carriers were loaded with USP14 siRNA to …

WebNov 1, 2024 · A549 Cells Aged Animals Antineoplastic Agents, Immunological / administration & dosage* Antineoplastic Agents, Immunological / pharmacology B7-H1 Antigen / genetics* B7-H1 Antigen / metabolism* Carcinoma, Non-Small-Cell Lung / drug therapy* Carcinoma, Non-Small-Cell Lung / genetics Carcinoma, Non-Small-Cell Lung / … WebJan 20, 2024 · The A549/DDP cells were plated at a density of 5 × 10 3 cells per well in a 96-well plate and cultured overnight. Then, the cells were transfected 24 h prior to glutamine deprivation with siRNA-targeting SDHB (stB0007812C, sequence 5’→3’: CCCGAAGGTTGACACCAA) or non-targeting control (RiboBio Technology) using …

WebSilencing USP14 increased the tumor antagonistic action of DDP in A549 cisplatin‐resistant (A549/DDP) cells, while USP14 overexpression decreased the antagonist effects. …

WebEffect of MiR-195-5p on the Sensitivity of A549 and A549/DDP Cells to Cisplatin Sensitivity to cisplatin in the different groups was measured as the IC 50 concentration after … eaoron where to buyWebBottom Line: Characterisation studies demonstrated a decreased proliferative capacity of lung tumour cells in response to cisplatin, increased resistance to cisplatin-induced cell death, accumulation of resistant cells in the G0/G1 phase of the cell cycle and enhanced clonogenic survival ability.The stem cell markers, Nanog, Oct-4 and SOX-2, were … eaoron tonerWebTargeted therapy for cisplatin‐resistant lung cancer via aptamer‐guided nano‐zinc carriers containing USP14 siRNA Xinmin Zhao,1 ,2 Xianghua Wu,1 ,2 Huijie Wang,1 ,2 Songtao Lai,2 ,3 ,4 and Jialei Wang1 ,2 Xinmin Zhao 1 Department of Thoracic Medical Oncology, Fudan University Shanghai Cancer Center, Shanghai China 2 Department of Oncology, eaoron ultra anti-wrinkle face serumWebThe IC 50 of cisplatin on A549 cells was 4.07±0.32, 2.26±0.43, and 1.52±0.41 µg/mL, for 24, 48, and 72 hours, respectively. Thus, we used 4, 2, and 1 µg/mL of cisplatin to treat A549 cells... eaoron 面膜WebApoptosis of Cisplatin-induced A549 cells was determined by immunofluorescence and flow cytometry, respectively. Western blot was performed to detect protein levels of Prx V, Bcl … eaoron ptt eaoron whitening creamWebWe previously had obtained cisplatin-resistant non-small cell lung cancer (NSCLC) A549 cell lines (A549/DDP) and its parental A549 cells. 12,13 In this study, we aim to observe the difference of autophagy between A549/DDP and A549, and also determine the role autophagy plays and the underlying regulation mechanisms during DDP treatment in … eaor phone