Cst tbk1 antibody
WebSep 14, 2024 · Lysates were incubated with anti-TBK1 rabbit monoclonal antibody (CST; Cat #3504) and 30 μL Protein A/G beads (Santa Cruz Biotechnology) overnight at 4°C. Immunoprecipitates were washed 3 times in IP lysis buffer plus 1 mmol/L PMSF then heated to ≥95°C for ≥5 minutes in standard SDS sample buffer. WebImmunohistochemical analysis of paraffin-embedded human breast carcinoma, using α-Tubulin Antibody in the presence of control peptide (left) or antigen-specific peptide (right). Confocal immunofluorescent …
Cst tbk1 antibody
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WebJul 18, 2024 · Antigen retrieval performed by microwave heating in citric acid antigen repair solution (pH = 6.0). All sections were incubated rabbit anti-p-TBK1 (1:100, CST) or rabbit …
WebPolyclonal Antibody for studying TBK1. Cited in 166 publications. Validated for Western Blotting, Immunoprecipitation. Highly specific and rigorously … WebApr 13, 2024 · A signaling cascade triggered by the STING-independent activation of TBK1 would ultimately lead to the P65 engagement (Cai et al. 2024). The activated STING-TBK1-P65 signaling pathway is associated with promoting innate immune response and necrosis of hepatic tissues, participating in multiple types of acute and chronic hepatic injury.
WebStat1 was detected using Stat1 Antibody #9172, and p42 was detected using p42 MAPK Antibody #9108. The Stat1 Antibody confirms silencing of Stat1 expression, and the p42 MAPK Antibody is used to control for … WebUpon activation by mitogenic and/or stress stimuli mediated by Erk and p38 MAPK, Mnk1 phosphorylates eIF4E at Ser209 in vivo (3,4). Two Erk and p38 MAPK phosphorylation sites in mouse Mnk1 (Thr197 and Thr202) are essential for Mnk1 kinase activity (3). The carboxy-terminal region of eIF4G also contains serum-stimulated phosphorylation sites ...
WebDec 10, 2015 · The two primary antibodies prepared in 5% normal goat serum solution to appropriate concentrations (3:100 of each antibody; TBK1 from Cell Signaling and CEP170 from Invitrogen) was added to …
WebAntibody Duet for studying TBK1 (Ser172) phosphate/TBK1 in the research area. ... PhosphoPlus ® Duets from Cell Signaling Technology (CST) provide a means to assess protein activation status. Each Duet contains an activation-state and total protein antibody to your target of interest. These antibodies have been selected from CST's product ... philosopher fallsWebSep 3, 2024 · Established in Beverly, MA in 1999, Cell Signaling Technology (CST) is a privately-owned company with over 400 employees worldwide. We are dedicated to providing innovative research tools that are used to help define mechanisms underlying cell function and disease. ... human TBK1 antibody; mouse Tbk1 antibody; rat Tbk1 … tsh and t4 testsWebDec 10, 2015 · Interaction between proteins in vivo was analysed by immunoprecipitation–western blot analyses with 250 μg of lysate and 1 μg of the … philosopher fichteWebBackground. The mitochondrial antiviral signaling protein (MAVS, VISA) contributes to innate immunity by triggering IRF-3 and NF-κB activation in response to viral infection, leading to the production of IFN-β (1). The MAVS protein contains an N-terminal CARD domain and a C-terminal mitochondrial transmembrane domain. philosopher femaleWebAll lanes : Anti-NAK/TBK1 (phospho S172) antibody [EPR2867(2)] (ab109272) at 1/5000 dilution Lane 1 : Untreated HeLa (Human cervix adenocarcinoma epithelial cell) whole … philosopher filterWebProduct Description. The Human Monocyte STING Activation Flow Cytometry Panel includes antibodies targeting phospho-STING (Ser366) and phenotyping markers to enable observation of STING activation in human monocyte populations among peripheral blood mononuclear cells (PBMCs). CD45 is a pan leukocyte marker. tsh and weight gainWebBinding of CDT1 to geminin prevents pre-RC formation, and expression and degradation of geminin serve to regulate CDT1 activity (reviewed in 5). The interaction of CDT1 with … tsh and trh