Phh cells
WebJan 1, 2024 · The above results suggested that 6:2 Cl-PFESA and PFOS could disrupt cellular metabolic processes but in distinct mechanisms in PHH cells, i.e., the disturbance effect induced by 6:2 Cl-PFESA might depend on cellular membrane-bounded signaling pathway, while the disturbance effect induced by PFOS might depend on intracellular transport … WebJul 22, 2024 · Background & aims: Pluripotent stem cell (PSC)-derived hepatocyte-like cells (HLC) have enormous potential as a replacement for primary hepatocytes in drug screening, toxicology and cell replacement therapy, but their genome-wide expression patterns differ strongly from primary human hepatocytes (PHH).
Phh cells
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WebJul 28, 2024 · The read number of the glycogenes from the RNA-Seq results of primary human hepatic (PHH) cells, HuH7 cells and HepG2 cells was used to calculate RPKM per cell line. The glycogenes are aligned from the highest to the lowest expression based on RPKM from PHH cells. Red indicates high expression levels while blue indicates low. WebApr 12, 2024 · Although PHH and HepaRG cells showed overlap in deregulated genes and modules by the liver toxicants, PHH demonstrated a higher responsiveness, based on the lower BMCs of co-regulated gene modules. Such BMCs can be used as point of departure (PoD) for assessing module-associated cellular (stress) pathways/processes.
WebOct 14, 2024 · Freshly isolated primary human hepatocytes (PHHs) represent the gold standard to investigate liver functions. However, standard two-dimensional PHH cultures are difficult to expand and rapidly... WebMar 6, 2014 · Fold induction in hiHep and PHH cells were normalized to the levels in cells without inducer treatment, respectively. (D) CYP metabolic activity in hiHeps. CYP enzymes were induced in hiHeps for 48 hr. Freshly thawed primary human hepatocytes were directly used as a positive control. The metabolic products of phenacetin (acetaminophen, assay ...
Web(a-b) HBV-infected PHH cells were treated with 7-aminoactinomycin D (5 μg/ml). Total HBV RNAs and 3.5-kb RNA were quantified by RT-qPCR and the amount of RNA at time zero was set at 100%. WebFeb 27, 2014 · hiHep cells can be used to treat acute liver failure and metabolic liver disease Summary The generation of large numbers of functional human hepatocytes for cell-based approaches to liver disease is an important and unmet goal.
WebNov 14, 2024 · Cell: HBV-infected PHH cells. Methods: PHH cells were infected with 2000 genome equivalents/cell of HBV particles in the presence of 4% PEG8000. Seven days …
WebAug 1, 2024 · Primary human hepatocytes (PHHs, Table 1) are considered the gold standard short-term human in vitro liver model because of their high functionality relative to the human organ in vivo. They can be obtained either from isolation of liver resections or liver tissues improper for transplantation or cryopreserved from commercial suppliers. foodinloveoutWebPrimary Human Hepatocytes (PHH) are considered the gold standard in vitro model for studying hepatic biology, liver function, and drug induced hepatotoxicity. However, PHH … elderly falling statisticsWebIntracellular pH ( pHi) is the measure of the acidity or basicity (i.e., pH) of intracellular fluid. The pHi plays a critical role in membrane transport and other intracellular processes. In an … food in louisville kentuckyWebMar 30, 2016 · PHH were separated from the NPC by an initial centrifugation at 50 x g. Density gradient centrifugation steps were used for removal of dead cells. Individual liver … food in louisville kyWebMay 4, 2016 · PHH spheroids can be successfully co-cultured with non-parenchymal Kupffer, stellate and biliary cells. Immunofluorescent stainings ( A) as well as qPCR analyses ( B) reveal the presence of... elderly falling preventionWebSep 25, 2015 · The obtained cell suspension was filtered through a 230μm-meshed cell strainer. PHH were then separated from NPC by low-speed centrifugation at gradually increasing rates (30×g, 40×g, and 50×g, for 10min). The cell pellets were resuspended in perfusion solution, whereas the supernatants were collected for the preparation of NPC, … elderly fall lift deviceWebHepaRG cells comprise a biologically relevant alternative model that exhibits many characteristics of primary human hepatocytes, including morphology and expression of key metabolic enzymes (Phase I and II), nuclear … elderly falls and death