Ptrc-hisa
WebMar 8, 2002 · We constructed an expression plasmid, using the vector (pTrc-HisA) and the monkey cDNA for the 290-amino-acid polypeptide. The recombinant protein with a M r of 33 kDa exhibited PGE synthase activity and was purified to apparent homogeneity by nickel-chelating column chromatography.
Ptrc-hisa
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Web1 hour ago · The fragment was cloned using BamH1 and EcoR1 (adding a stop codon) into pTrcHisA. For fusion protein production, the DNA was transformed into BL21(DE3) competent cells (Invitrogen), induced mid ... Web淼灵 P0813 pTrcHisA ￥699.00 ￥699.00 丰晖 ZT222 PFastBacHTA ￥576.00 ￥691.20 淼灵 P0860 pCMV-FLAG-N; 商品介绍; 商品评论; 商品咨询; 加入购物车 ...
http://www.biofeng.com/zaiti/dachang/pTrcHisA.html WebThe wells containing bilayers were washed with RPMI culture media and warmed to 37°C for 30 min prior to adding the cells. Cells were added to the bilayer at a density of 250,000 cells/well for 4–5 min at 37°C and fixed using 4% PFA in PBS warmed to 37°C. Fixation of cells was done for 15 min at 37°C.
WebProtein Expression Vectors. Select from a variety of protein expression vectors for expressing cloned sequences in bacterial and mammalian cells as well as cell-free systems. Use the multi-purpose Flexi® Vectors for easy cloning and transfer of ORFs, or choose products for regulated expression of proteins and identifying protein interactions ... WebPublic Transport Regulatory Commission (Jordan) PTRC. Petroleum Technology Research Center. PTRC. Protective Technology Research Centre (Nanyang Technological …
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WebBacterial vector for inducible expression of N-terminally 6xHis-tagged proteins. For other reading frames, use pTrcHis B or pTrcHis C. thailand\u0027s loi krathongWebNational Center for Biotechnology Information thailand\\u0027s loi krathong rhythmWebDownload the RaiseRight app and get egift cards sent to you directly. TO ORDER ONLINE. PCHS PTSA Scholarships. Apply for one of our three $500 scholarships to be applied to … thailand\\u0027s locationWebThe microarray experiments with L133 (ramA::aph) and L786 (L133 pTRChisA-ramA) revealed significantly altered expres- sion of Salmonella pathogenicity island genes. Inactivation of ramA increased ... thailand\\u0027s loi krathong melodyWebApr 1, 2024 · To better understand the function of BoPAL proteins, the four BoPAL genes were subcloned into pTrcHisA plasmids (Table S2) and heterologously expressed in the E. coli Top10 strain. A 2.2 kb BoPAL3 fragment was confirmed by PCR using pTrcHisA-BoPAL3 plasmid as template (Fig. 1B). Recombinant BoPAL protein affinity purified by Ni resin … synchrotron vs cyclotronWebDec 12, 2008 · The PRH (proline-rich homeodomain) [also known as Hex (haematopoietically expressed homeobox)] protein is a transcription factor that functions as an important regulator of vertebrate development and many other processes in the adult including haematopoiesis. The Groucho/TLE (transducin-like enhancer) family of co-repressor … synchrotron usesWebpTrcHisA Description pTrcHisA vector is an expression vector derived from pBR322 plasmids and is designed to express and purify the recombinant protein in Escherichia … thailand\\u0027s main exports